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Phospho-TSC2 (Ser939) Rekombinanter Antikörper

Phospho-TSC2 (Ser939) Rekombinant Antikörper für FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2B18

Kat-Nr. : CL647-81654

Synonyme

p TSC2, p TSC2 (Ser939), p TSC2 Ser939, Phospho TSC2, Phospho TSC2 (Ser939)

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 647-conjugated Phospho-TSC2 (Ser939 (CL647-81654)

    FC experiment of HEK-293 using CL647-81654

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Tsc2 (Ser939) Recombinant Antibody (CL647-81654, Clone:2B18)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Geprüfte Anwendungen

Erfolgreiche Detektion in FC (Intra)Mit Calyculin A behandelte HEK-293-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL647-81654 bindet in FC (Intra) Phospho-TSC2 (Ser939) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name tuberous sclerosis 2
Berechnetes Molekulargewicht 1807 aa, 201 kDa
Beobachtetes Molekulargewicht200 kDa
GenBank-ZugangsnummerBC150300
Gene symbol TSC2
Gene ID (NCBI) 7249
Konjugation CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).