Phospho-ULK1 (Ser556) Rekombinanter Antikörper

Phospho-ULK1 (Ser556) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F5

Kat-Nr. : 80218-1-PBS

Synonyme

ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

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  WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

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  WB analysis using 80218-1-RR (same clone as 80218-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 80218-1-RR (same clone as 80218-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-ULK1 (Ser556) (80218-1-RR, Clone:2F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80218-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

80218-1-PBS bindet in WB, Indirect ELISA Phospho-ULK1 (Ser556) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)