Phospho-ULK1 (Ser556) Rekombinanter Antikörper

Phospho-ULK1 (Ser556) Rekombinant Antikörper für WB, ELISA, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(23)

CloneNo.

2F5

Kat-Nr. : 80218-1-RR

Synonyme

ULK1, KIAA0722, hATG1, EC:2.7.11.1, Autophagy-related protein 1 homolog

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  WB analysis using 80218-1-RR

  Non-treated and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control.

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  WB analysis using 80218-1-RR

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80218-1-RR (Phospho-ULK1 (Ser556) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with mouse anti-mTOR antibody 66888-1-Ig as loading control.

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  FC experiment of PC-3 using 80218-1-RR

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.5 ug Anti-Human Phospho-ULK1 (Ser556) (80218-1-RR, Clone:2F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.5 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, Mit Calyculin A behandelte HeLa-Zellen, Mit Calyculin A behandelte PC-3-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): Mit Calyculin A behandelte PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

80218-1-RR bindet in WB, IHC, IF, FC (Intra), ELISA Phospho-ULK1 (Ser556) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367)

Publikationen

Species	Application	Title
human	WB	J Hazard Mater; Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.; Authors - Feng Xiaoli
mouse	WB	JCI Insight; ZNFX1 promotes AMPK-mediated autophagy against Mycobacterium tuberculosis by stabilizing mRNA; Authors - Honglin Liu
human	WB	Sci Rep; EYA3 promotes the tumorigenesis of gastric cancer through activation of the mTORC1 signaling pathway and inhibition of autophagy; Authors - Zhen Xu
human	WB	Int J Gen Med; Sigma-1 Receptor Rescues Autophagy Through AMPK/mTOR Signaling Pathway in Sepsis-Induced Acute Kidney Injury; Authors - Wei Jiang
human	WB	Bioengineered; Shikonin induces apoptosis and autophagy via downregulation of pyrroline-5-carboxylate reductase1 in hepatocellular carcinoma cells.; Authors - Junli Zhang
mouse	IF	bioRxiv; AMPK is dispensable for physiological podocyte and glomerular functions but prevents glomerular fibrosis in experimental diabetes; Authors - Swayam Prakash Srivastava