Phospho-ULK1 (Ser758) Rekombinanter Antikörper

Phospho-ULK1 (Ser758) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240770A7

Kat-Nr. : 82776-8-PBS

Synonyme

P ULK1 (Ser758), Phos ULK1 (Ser758), Phospho-ULK1 (Ser757), Phos-ULK1 (Ser758), P-ULK1 (Ser758)

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  WB analysis using 82776-8-RR (same clone as 82776-8-PBS)

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control. This data was developed using the same antibody clone with 82776-8-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82776-8-PBS bindet in WB, Indirect ELISA Phospho-ULK1 (Ser758) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: unc-51-like kinase 1 (C. elegans)
• Beobachtetes Molekulargewicht: 140 kDa
• GenBank-Zugangsnummer: NM_003565
• Gene symbol: ULK1
• Gene ID (NCBI): 8408
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367).This antibody is equivalent to Ser757 in mice.