Phospho-XIAP (Ser87) Polyklonaler Antikörper

Phospho-XIAP (Ser87) Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28791-1-PBS

Synonyme

---

• 

  WB analysis using 28791-1-AP (same clone as 28791-1-PBS)

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28791-1-AP (Phospho-XIAP (Ser87) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28791-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

28791-1-PBS bindet in WB, Indirect ELISA Phospho-XIAP (Ser87) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: X-linked inhibitor of apoptosis
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC032729
• Gene symbol: XIAP
• Gene ID (NCBI): 331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. XIAP is directly phosphorylated by PKC and that XIAP phosphorylated at Ser87 by PKC is resistant to degradation, protecting cells from apoptosis-inducing stress.