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Phospho-YAP1 (Ser397) Rekombinanter Antikörper

Phospho-YAP1 (Ser397) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250306F11

Kat-Nr. : 80694-3-PBS

Synonyme

p YAP1 (Ser397), Phospho YAP1 (Ser397), Phospho-YAP, Phospho-YAP (Ser397), Phospho-YAP(Ser397)

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-YAP1 (Ser397) Recombinant antibody (80694-3-RR)

    WB analysis of NIH/3T3 using 80694-3-RR (same clone as 80694-3-PBS)

    NIH/3T3 cell lysates were subjected to SDS PAGE followed by western blot with 80694-3-RR (Phospho-YAP1 (Ser397) antibody) blocked with BSA only, Phospho-YAP1 (Ser397) peptide or non-Phospho peptide at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80694-3-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80694-3-PBS bindet in WB, Indirect ELISA Phospho-YAP1 (Ser397) und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name Yes-associated protein 1, 65kDa
Berechnetes Molekulargewicht 504 aa, 54 kDa
Beobachtetes Molekulargewicht75 kDa
GenBank-ZugangsnummerBC038235
Gene symbol YAP1
Gene ID (NCBI) 10413
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)