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Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Rekombinanter Antikörper

Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250683A9

Kat-Nr. : 81767-2-PBS

Synonyme

Phospho Syk (Tyr352), Phospho ZAP70 (Tyr319)/Syk (Tyr352), Phospho Zap-70 (Tyr319)/Syk (Tyr352), Phospho-Syk (Tyr352), Phospho-Syk-Tyr352

Filter:
  • Western Blot (WB) analysis of Jurkat cells using Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Recombinant mo (81767-2-RR)

    WB analysis of Jurkat using 81767-2-RR (same clone as 81767-2-PBS)

    Non-treated Jurkat cells and H2O2 treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81767-2-RR (Phospho-ZAP70 (Tyr319)/SYK (Tyr352) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81767-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

81767-2-PBS bindet in WB, Indirect ELISA Phospho-ZAP70 (Tyr319)/SYK (Tyr352) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name zeta-chain (TCR) associated protein kinase 70kDa
Berechnetes Molekulargewicht 70 kDa
Beobachtetes Molekulargewicht70 kDa, 72kDa
GenBank-ZugangsnummerBC039039
Gene symbol ZAP70
Gene ID (NCBI) 7535
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. Three tyrosine residues (Tyr292, Tyr315 and Tyr319) in the interdomain B region of ZAP‐70 have attracted particular attention as sites that might regulate ZAP‐70 activity and/or binding to heterologous proteins. The third candidate phosphorylation site in the interdomain B region of ZAP‐70 is Tyr319. Although the functional importance of this site in ZAP‐70 is unknown, a previous report presented indirect evidence that the orthologous Tyr352 residue in Syk is involved in the association of PLC‐γ1 with this PTK. (PMID: 10202147)