Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Rekombinanter Antikörper

Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250683A9

Kat-Nr. : 81767-2-RR

Synonyme

Phospho Syk (Tyr352), Phospho ZAP70 (Tyr319)/Syk (Tyr352), Phospho Zap-70 (Tyr319)/Syk (Tyr352), Phospho-Syk (Tyr352), Phospho-Syk-Tyr352

---

• 

  WB analysis of Jurkat using 81767-2-RR

  Non-treated Jurkat cells and H2O2 treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81767-2-RR (Phospho-ZAP70 (Tyr319)/Syk (Tyr352) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: H2O2 treated Jurkat cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81767-2-RR bindet in WB, ELISA Phospho-ZAP70 (Tyr319)/SYK (Tyr352) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: zeta-chain (TCR) associated protein kinase 70kDa
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa, 72kDa
• GenBank-Zugangsnummer: BC039039
• Gene symbol: ZAP70
• Gene ID (NCBI): 7535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. Three tyrosine residues (Tyr292, Tyr315 and Tyr319) in the interdomain B region of ZAP‐70 have attracted particular attention as sites that might regulate ZAP‐70 activity and/or binding to heterologous proteins. The third candidate phosphorylation site in the interdomain B region of ZAP‐70 is Tyr319. Although the functional importance of this site in ZAP‐70 is unknown, a previous report presented indirect evidence that the orthologous Tyr352 residue in Syk is involved in the association of PLC‐γ1 with this PTK. (PMID: 10202147)