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Phospho-c-SRC (Tyr530) Rekombinanter Antikörper

Phospho-c-SRC (Tyr530) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241477A4

Kat-Nr. : 80706-2-PBS

Synonyme

c-SRC, SRC, p60-Src, EC:2.7.10.2, cSRC

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-c-SRC (Tyr530) Recombinant antibody (80706-2-RR)

    WB analysis using 80706-2-RR (same clone as 80706-2-PBS)

    Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80706-2-RR (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80706-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80706-2-PBS bindet in WB, Indirect ELISA Phospho-c-SRC (Tyr530) und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
Berechnetes Molekulargewicht 60 kDa
Beobachtetes Molekulargewicht60 kDa
GenBank-ZugangsnummerBC011566
Gene symbol SRC
Gene ID (NCBI) 6714
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Src is a 60 kDa protein encoded by the Src gene belonging to a family of non-RTK known as SFK. As a typical protein, the polypeptide sequence of c-Src comprises an N-terminal head and a C-terminal tail but has four Src homology (SH) domains numbered 1, 2, 3, and 4. The C-terminal tail consists of a carboxy group that is short and connected to the SH1 domain. Additionally, the C-terminal domain serves as an anchor for a phosphotyrosine moiety, specifically Tyr530 in human Src (or Tyr527 in chicken). (PMID: 38201459)