Phospho-c-SRC (Tyr530) Rekombinanter Antikörper

Phospho-c-SRC (Tyr530) Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

241477A4

Kat-Nr. : 80706-2-RR

Synonyme

c-SRC (Tyr530), p c-SRC (Tyr530), p-c-SRC (Tyr530), Phospho c-SRC (Tyr530), Phospho-SRC (Tyr530)

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  WB analysis using 80706-2-RR

  Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 80706-2-RR (c-SRC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit LPS behandelte THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

80706-2-RR bindet in WB, ELISA Phospho-c-SRC (Tyr530) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC011566
• Gene symbol: SRC
• Gene ID (NCBI): 6714
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

c-Src is a 60 kDa protein encoded by the Src gene belonging to a family of non-RTK known as SFK. As a typical protein, the polypeptide sequence of c-Src comprises an N-terminal head and a C-terminal tail but has four Src homology (SH) domains numbered 1, 2, 3, and 4. The C-terminal tail consists of a carboxy group that is short and connected to the SH1 domain. Additionally, the C-terminal domain serves as an anchor for a phosphotyrosine moiety, specifically Tyr530 in human Src (or Tyr527 in chicken). (PMID: 38201459)

Publikationen

Species	Application	Title
mouse,human	WB	J Ethnopharmacol; Paeoniflorin inhibits chronic restraint stress-induced progression of hepatocellular carcinoma through suppressing norepinephrine-induced activation of hepatic stellate cells via SRC/AKT/ERK pathways; Authors - Yujun Luo
mouse	WB	Neurol Res; Exploring neuroprotective effects of PP2 in ischemic stroke via bioinformatics and experimental validation; Authors - Shiyan Zhao