Phospho-mTOR (Ser2481) Polyklonaler Antikörper

Phospho-mTOR (Ser2481) Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus und mehr (2)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(11)

Kat-Nr. : 28879-1-AP

Synonyme

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1

---

• 

  WB analysis using 28879-1-AP

  Nontreated and insulin-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28879-1-AP (Phospho-mTOR (Ser2481) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Insulin behandelte NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below

Produktinformation

28879-1-AP bindet in WB, ELISA Phospho-mTOR (Ser2481) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: FK506 binding protein 12-rapamycin associated protein 1
• Berechnetes Molekulargewicht: 289 kDa
• Beobachtetes Molekulargewicht: 250-289 kDa
• GenBank-Zugangsnummer: BC117166
• Gene symbol: mTOR
• Gene ID (NCBI): 2475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MTOR, also named FRAP1, FRAP, FRAP2, and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is the kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. mTORC2 is also activated by growth factors but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

Publikationen

Species	Application	Title
human	WB	Anal Cell Pathol (Amst); Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis; Authors - Junli Zhang
rat	WB	Am J Transl Res; Integrating network pharmacology with experimental validation to investigate the mechanism of Wuwei Zishen formula in improving perimenopausal syndrome; Authors - Xuewen Fu
human	WB	Naunyn Schmiedebergs Arch Pharmacol; Network pharmacology-based strategy to reveal the mechanism of pinocembrin against ovarian cancer; Authors - Guanghui Wang
human	WB	Sci Rep; Morphine promotes non-small cell lung cancer progression by downregulating E-cadherin via the PI3K/AKT/mTOR pathway; Authors - Fulei Gu
mouse	WB	Int J Biol Macromol; Lactoferrin ameliorates cognitive impairment in D-galactose-induced aging mice by regulating the PI3K/Akt/mTOR signaling pathway and the microbiome-gut-brain axis; Authors - Mengqi Wang
mouse	WB	Eur J Neurosci; Oleracein E Rejuvenates Senescent Hippocampal NSCs by Inhibiting the ERK1/2-mTOR Axis to Improve Cognitive Dysfunction in Vascular Dementia; Authors - Haili Lang