Phospho-p38 MAPK (Thr180/Tyr182) Rekombinanter Antikörper

Phospho-p38 MAPK (Thr180/Tyr182) Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242308D3

Kat-Nr. : 81212-2-PBS

Synonyme

p38 (Thr180/Tyr182), p38 MAPK (Thr180/Tyr182), phospho-p38, phospho-p38 (Thr180/Tyr182), Phospho-p38 MAPK

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  WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

  Non-treated NIH/3T3 cells, UV treated and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

  Non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81212-2-RR (same clone as 81212-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

81212-2-PBS bindet in WB, IHC, Indirect ELISA Phospho-p38 MAPK (Thr180/Tyr182) und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: mitogen-activated protein kinase 14
• Berechnetes Molekulargewicht: 360 aa, 41 kDa
• Beobachtetes Molekulargewicht: 38-42 kDa
• GenBank-Zugangsnummer: BC031574
• Gene symbol: p38 MAPK
• Gene ID (NCBI): 1432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)