Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) Rekombinanter Antikörper

Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G15

Kat-Nr. : 82373-1-PBS

Synonyme

p p70(S6K), p p70(S6K) (Thr389), p p70(S6K) Thr389, P p85(S6K) (Thr412), p70(S6K) (Thr389)

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  WB analysis of MCF-7 using 82373-1-RR (same clone as 82373-1-PBS)

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82373-1-RR (Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta actin antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 82373-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

82373-1-PBS bindet in WB, Indirect ELISA Phospho-p70(S6K) (Thr389)/p85(S6K) (Thr412) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Beobachtetes Molekulargewicht: 65-85 kDa
• GenBank-Zugangsnummer: NM_003161
• Gene symbol: p70 S6K
• Gene ID (NCBI): 6198
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70(S6K) plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85(S6K) and p70(S6K), which is identical to p85(S6K) but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). mTOR is know to phosphorylate and thereby activate p70(S6K) at Thr389 and p85(S6K) at Thr412. (PMID: 25100792, PMID: 24970012, PMID: 21602892)