Phospholipase C epsilon 1 Polyklonaler Antikörper

Phospholipase C epsilon 1 Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 55463-1-AP

Synonyme

KIAA1516, Phospholipase C epsilon 1, phospholipase C, epsilon 1, PLC epsilon 1, PLCE, PLCE1, PPLC

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Galerie der Validierungsdaten

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  IHC staining of human kidney using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human oesophagus cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 40x lens).

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  IHC staining of human stomach cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 10x lens).

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  IHC staining of human stomach cancer using 55463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55463-1-AP (Phospholipase C epsilon 1 antibody at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Kolongewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Magenkrebsgewebe, humanes Ösophaguskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

55463-1-AP bindet in IHC, ELISA Phospholipase C epsilon 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: phospholipase C, epsilon 1
• Berechnetes Molekulargewicht: 257 kDa
• GenBank-Zugangsnummer: NM_016341
• Gene symbol: PLCE1
• Gene ID (NCBI): 51196
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Phospholipase C epsilon 1 (PLCE1) is also named as KIAA1516, PLCE and PPLC. TRAPPC10 (Cyclin-J) Belongs to the Cyclin family. PLCE1, which mediates small GTPases of the Ras superfamily through the activity of its Ras guanine exchange factor, is a bifunctional enzyme (PMID: 31867717). As the effector of heterotrimer and small G protein, it is involved in regulating cell growth, cell survival, T-cell activation, and actin organization (PMID: 32238860). PLCE1 is identified as a member of phospholipase family, and PLCE1 was upregulated in LPS-induced pneumonia cells (PMID: 35527658). PLCE1 is involved in the regulation of Rho GTPases and MAPKs in podocytes, and PLCE1 interacts with NCK2 but not with NCK1 (PMID: 32238860). PLCE1 is a multifunctional enzyme exhibiting phospholipase as well as RasGEF activities (PMID: 11022047).