Piezo1 (extracellular domain) Rekombinanter Antikörper

Piezo1 (extracellular domain) Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4B15

Kat-Nr. : 82625-4-PBS

Synonyme

FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment

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  WB analysis using 82625-4-RR (same clone as 82625-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Ag7791 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

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  WB analysis using 82625-4-RR (same clone as 82625-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

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  IHC staining of rat dorsal root ganglion using 82625-4-RR (same clone as 82625-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82625-4-RR (Piezo1 (extracellular domain) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 82625-4-RR (same clone as 82625-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using Piezo1 (extracellular domain) antibody (82625-4-RR, Clone: 4B15 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82625-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82625-4-RR

  Biolayer interferometry (BLl) kinetic assays of 82625-4-RR against Human Piezo1 (extracellular domain) were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82625-4-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Piezo1 (extracellular domain) und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Piezo1 (extracellular domain) fusion protein Ag7791
• Vollständiger Name: family with sequence similarity 38, member A
• Berechnetes Molekulargewicht: 286 kDa
• Beobachtetes Molekulargewicht: 233-286 kDa
• GenBank-Zugangsnummer: BC008073
• Gene symbol: Piezo1/FAM38A
• Gene ID (NCBI): 9780
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)