Piezo1 (extracellular domain) Rekombinanter Antikörper

Piezo1 (extracellular domain) Rekombinant Antikörper für IF/ICC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B15

Kat-Nr. : CL488-82625-4

Synonyme

FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment

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  IF Staining of THP-1 using CL488-82625-4

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 Piezo1 (extracellular domain) antibody (CL488-82625-4, Clone: 4B15 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: THP-1-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-82625-4 bindet in IF/ICC Piezo1 (extracellular domain) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Piezo1 (extracellular domain) fusion protein Ag7791
• Vollständiger Name: family with sequence similarity 38, member A
• Berechnetes Molekulargewicht: 286 kDa
• Beobachtetes Molekulargewicht: 233-286 kDa
• GenBank-Zugangsnummer: BC008073
• Gene symbol: Piezo1/FAM38A
• Gene ID (NCBI): 9780
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)