Pikachurin Monoklonaler Antikörper

Pikachurin Monoklonal Antikörper für WB, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C6D12

Kat-Nr. : 66232-1-PBS

Synonyme

EGFLAM, 1C6D12, Agrin like protein, AGRINL, Agrin-like protein

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  WB analysis of human retina using 66232-1-Ig (same clone as 66232-1-PBS)

  human retina tissue were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 66232-1-Ig (same clone as 66232-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 66232-1-Ig (Pikachurin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 66232-1-Ig (same clone as 66232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

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  IF Staining of Y79 using 66232-1-Ig (same clone as 66232-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Y79 cells using Pikachurin antibody (66232-1-Ig, Clone: 1C6D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66232-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66232-1-PBS bindet in WB, IF/ICC, IF-P, Indirect ELISA Pikachurin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Pikachurin fusion protein Ag6362
• Vollständiger Name: EGF-like, fibronectin type III and laminin G domains
• Berechnetes Molekulargewicht: 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa, 55-60 kDa
• GenBank-Zugangsnummer: BC033177
• Gene symbol: Pikachurin
• Gene ID (NCBI): 133584
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).