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Pinin Rekombinanter Antikörper

Pinin Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251028A12

Kat-Nr. : 86292-1-PBS

Synonyme

140 kDa nuclear and cell adhesion-related phosphoprotein, Desmosome-associated protein, Domain-rich serine protein, DRS, PNN

Filter:
  • Western Blot (WB) analysis of various lysates using Pinin Recombinant antibody (86292-1-RR)

    WB analysis using 86292-1-RR (same clone as 86292-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86292-1-RR (PNN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86292-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Pinin Recombinant antibody (86292-1-RR)

    IF Staining of HepG2 using 86292-1-RR (same clone as 86292-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PNN antibody (86292-1-RR, Clone: 251028A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86292-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86292-1-PBS bindet in WB, IF/ICC, Indirect ELISA Pinin und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Pinin fusion protein Ag38940
Vollständiger Name pinin, desmosome associated protein
Berechnetes Molekulargewicht 82 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC062602
Gene symbol Pinin
Gene ID (NCBI) 5411
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PNN, also named as DRS or 140 kDa nuclear and cell adhesion-related phosphoprotein, is a 717 amino acid protein, which belongs to the pinin family. PNN localizes in the plasma membrane and is expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. PNN is expressed strongly in melanoma metastasis lesions and advanced primary tumors. PNN as a transcriptional activator binds to the E-box 1 core sequence of the E-cadherin promoter gene. PNN is involved in the establishment and maintenance of epithelia cell-cell adhesion. PNN is a potential tumor suppressor for renal cell carcinoma. The calculated molecular weight of PNN is 82 kDa (native form), but the post-modified protein is 140 kDa (phospho form of isoform 1).