Gamma Catenin Monoklonaler Antikörper

Gamma Catenin Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Hund, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

1A5D10

Rezensionen (1)

Kat-Nr. : 66445-1-Ig

Synonyme

JUP, JUP/gamma Catenin, γ-Catenin, ARVD12, Catenin gamma

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  WB analysis of human heart using 66445-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66445-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 66445-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of T-47D using 66445-1-Ig

  T-47D cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 66445-1-Ig

  A431 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 66445-1-Ig

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 66445-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDCK using 66445-1-Ig

  MDCK cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 66445-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of A431 using 66445-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of MCF-7 using 66445-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Herzgewebe, A431-Zellen, HT-29-Zellen, MDCK-Zellen, Rattenherzgewebe, T-47D-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Hautgewebe, humanes Magenkrebsgewebe, Maus-Magengewebe, Ratten-Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

66445-1-Ig bindet in WB, IHC, IF/ICC, ELISA Gamma Catenin und zeigt Reaktivität mit Hausschwein, human, Hund, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Hund, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gamma Catenin fusion protein Ag1627
• Vollständiger Name: junction plakoglobin
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 82 kDa
• GenBank-Zugangsnummer: BC011865
• Gene symbol: Gamma Catenin
• Gene ID (NCBI): 3728
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.

Publikationen

Species	Application	Title
human	WB	Aging (Albany NY); DSC2 suppresses the growth of gastric cancer through the inhibition of nuclear translocation of γ-catenin and PTEN/PI3K/AKT signaling pathway; Authors - Chao Sun
human	IF	Int J Mol Sci; The Non-Antibacterial Effects of Azithromycin and Other Macrolides on the Bronchial Epithelial Barrier and Cellular Differentiation; Authors - Arni Asbjarnarson
human	WB	Oxid Med Cell Longev; DSC2 Suppresses the Metastasis of Gastric Cancer through Inhibiting the BRD4/Snail Signaling Pathway and the Transcriptional Activity of β-Catenin; Authors - Chao Sun

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-02-2023)

Multiple bands and high background in western blot (1:2000, overnight incubation at 4C in 3% BSA PBS 0.1% Tween on PVDF membrane). Non-specific (and non-junctional) signalling in immunoflouresence (1:200, 4% PFA fixed cells).

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:2000 (WB) , 1:200 (IF)
• Cell Tissue Type: HUVEC

Gamma Catenin Antibody Western Blot,Immunofluorescence validation (1:2000 (WB) , 1:200 (IF) dilution) in HUVEC (Cat no:66445-1-Ig)