Plakophilin 1 Rekombinanter Antikörper

Plakophilin 1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242834G8

Kat-Nr. : 85217-4-RR

Synonyme

PKP1, 242834G8, B6P, Plakophilin-1

---

• 

  WB analysis using 85217-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 85217-4-RR

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 85217-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85217-4-RR against Human Plakophilin 1 were performed. The affinity constant is 11.4 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushautgewebe, A431-Zellen, HEK-293-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85217-4-RR bindet in WB, ELISA Plakophilin 1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Plakophilin 1 fusion protein Ag18153
• Vollständiger Name: plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
• Berechnetes Molekulargewicht: 747 aa, 83 kDa
• Beobachtetes Molekulargewicht: 70-75 kDa
• GenBank-Zugangsnummer: BC114571
• Gene symbol: PKP1
• Gene ID (NCBI): 5317
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).