Plasminogen Monoklonaler Antikörper

Plasminogen Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F6A11

Kat-Nr. : 66399-1-PBS

Synonyme

PLG, 1F6A11, Angiostatin, EC:3.4.21.7, Plasmin heavy chain A, short form

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  WB analysis of human plasma using 66399-1-Ig (same clone as 66399-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 66399-1-Ig (PLG Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66399-1-Ig (PLG Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66399-1-Ig (same clone as 66399-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Plasminogen antibody (66399-1-Ig, Clone: 1F6A11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66399-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66399-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA Plasminogen und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Plasminogen fusion protein Ag9938
• Vollständiger Name: plasminogen
• Berechnetes Molekulargewicht: 810 aa, 91 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC060513
• Gene symbol: Plasminogen
• Gene ID (NCBI): 5340
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Plasminogen is a secreted blood zymogen that is activated by proteolysis and converted to plasmin and angiostatin. Plasminogen plays an important role in fibrinolysis as well as wound healing, cell migration, tissue modeling and angiogenesis. Plasminogen is synthesized in the liver, and it circulates in the blood, with a half-life of 2.2 days. Plasminogen is the precursor of plasmin, which lyses fibrin clots to fibrin degradation products (FDP) and D-dimer; the conversion to active protease is mediated by tissue-type (tPA) and urokinase-type (uPA) plasminogen activators. Defects in plasminogen are likely a cause of thrombophilia and ligneous conjunctivitis.