Plexin B2 Polyklonaler Antikörper

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• KD/KO Validated

Plexin B2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10602-1-PBS

Synonyme

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• 

  WB analysis of A549 using 10602-1-AP (same clone as 10602-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 10602-1-AP (same clone as 10602-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10602-1-AP (same clone as 10602-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 10602-1-AP (same clone as 10602-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 10602-1-AP (same clone as 10602-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10602-1-AP (same clone as 10602-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 10602-1-AP (same clone as 10602-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

  IP result of anti-Plexin B2 (IP:10602-1-AP, 4ug; Detection:10602-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 10602-1-AP (Plexin B2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10602-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA Plexin B2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Plexin B2 fusion protein Ag0941
• Vollständiger Name: plexin B2
• Berechnetes Molekulargewicht: 205 kDa
• Beobachtetes Molekulargewicht: 80 kDa, 240 kDa
• GenBank-Zugangsnummer: BC004542
• Gene symbol: PLXNB2
• Gene ID (NCBI): 23654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.