Podocalyxin Monoklonaler Antikörper

Podocalyxin Monoklonal Antikörper für IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4A3C7

Kat-Nr. : 68250-1-PBS

Synonyme

PODXL, 4A3C7, GCTM 2 antigen, GCTM-2 antigen, Gp200

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  IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68250-1-PBS bindet in IHC, IF-P, Indirect ELISA Podocalyxin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Podocalyxin fusion protein Ag12859
• Vollständiger Name: podocalyxin-like
• Berechnetes Molekulargewicht: 526 aa, 55 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC093730
• Gene symbol: Podocalyxin
• Gene ID (NCBI): 5420
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).