Podoplanin Rekombinanter Antikörper

Podoplanin Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251406D4

Kat-Nr. : 86750-1-PBS

Synonyme

PDPN, 29kDa cytosolic podoplanin intracellular domain, Aggrus, PA2.26 antigen, PSEC0003

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  Sandwich ELISA sample test of MP02738-1

  Plasma of eight individual healthy human donors was measured. The Podoplanin concentration of detected samples was determined to be 85.9 pg/mL with a range of 37.1-122.1 pg/mL.

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  Sandwich ELISA standard curve of MP02738-1

  Sandwich ELISA standard curve of MP02738-1, Human Podoplanin Recombinant Matched Antibody Pair - PBS only. 86750-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg5024. 86750-5-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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Geprüfte Anwendungen

Produktinformation

86750-1-PBS bindet in Sandwich ELISA, Indirect ELISA Podoplanin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Podoplanin fusion protein Eg5024
• Vollständiger Name: podoplanin
• GenBank-Zugangsnummer: BC014668
• Gene symbol: Podoplanin
• Gene ID (NCBI): 10630
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Podoplanin was identified as a 43 kDa glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as a marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility, and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.