Polyglutamine Monoklonaler Antikörper

Polyglutamine Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

n/a

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

MW1

Kat-Nr. : 65239-1-Ig

Synonyme

Glutamine repeats, polyQ

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  WB analysis of Recombinant protein using 65239-1-Ig

  Western blot analysis of anti-polyglutamine antibody (MW1) binding to huntingtin exon 1 fusion proteins with variable numbers of glutamines. MW1 bound to huntingtin exon 1 proteins with normal and expanded polyQ repeats but did not bind the TRX tag control. (Owens, Gwen E et al. J Mol Biol. 2015 Jul 31;427(15):2507-2519.)

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  ELISA experiment of Recombinant protein using 65239-1-Ig

  Indirect ELISA was carried out by coating polyglutamine containing ASCL1 fusion protein (Cat.NO. Ag26337, containing polyglutamine region) and GST fusion protein expressed from the same vector (Cat.NO. Ag25094, for control) at 70 ng/well followed by blocking and adding serial diluted Polyglutamine mouse monoclonal antibody 65239-1-Ig. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Recombinant protein
• Erfolgreiche Detektion in ELISA: Recombinant protein

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:1000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

65239-1-Ig bindet in WB, ELISA Polyglutamine und zeigt Reaktivität mit n/a

• Getestete Reaktivität: n/a
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Polyglutamine
• GenBank-Zugangsnummer: N/A
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Huntington's disease is a neurodegenerative disorder caused by the expansion of a polyglutamine (polyQ) repeat in the N-terminal portion of huntingtin protein to a length above 35-40 units (PMID: 26047735; 19507258). The mutational expansion of polyglutamine above a critical length causes a toxic gain of function in huntingtin and results in neuronal death. In the course of the disease, expanded huntingtin is proteolyzed, becomes abnormally folded, and accumulates in oligomers, fibrils, and microscopic inclusions (PMID: 25336039). The anti-polyglutamine (polyQ) antibody MW1 specifically binds the polyQ domain of huntingtin exon 1. On western blot, the MW1 clone strongly prefers to bind to the expanded polyQ repeat form of Htt, displaying no detectable binding to normal huntingtin (PMID: 11719267).