PON2 Rekombinanter Antikörper

PON2 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

242673H1

Kat-Nr. : 85126-4-PBS

Synonyme

242673H1, A-esterase 2, Aromatic esterase 2, EC:3.1.1.81, PON 2

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  WB analysis using 85126-4-RR (same clone as 85126-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85126-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85126-4-RR (same clone as 85126-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85126-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01843-1

  Sandwich ELISA standard curve of MP01843-1, Mouse Pon2 Recombinant Matched Antibody Pair - PBS only. 85126-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2741. 85126-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP01843-1

  The mean Pon2 concentration was determined to be 344.03 ng/mL in mouse liver tissue extract based on a 3.70 mg/mL extract load, 812.45 ng/mL in rat liver tissue extract based on a 2.60 mg/mL extract load and 19.36 ng/mL in NIH/3T3 cell extract based on a 1.50 mg/mL extract load.

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  Cytometric bead array standard curve of MP01843-1

  Cytometric bead array standard curve of MP01843-1, MOUSE Pon2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85126-4-PBS. Detection antibody: 85126-2-PBS. Standard: Eg2741. Range: 1.563-200 ng/mL.

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Geprüfte Anwendungen

Produktinformation

85126-4-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PON2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PON2 fusion protein Eg2741
• Vollständiger Name: paraoxonase 2
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: NM_183308.2
• Gene symbol: Pon2
• Gene ID (NCBI): 330260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).