Pon3 Rekombinanter Antikörper

Pon3 Rekombinant Antikörper für WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251535H2

Kat-Nr. : 86577-1-PBS

Synonyme

EC:3.1.8.1, Paraoxanase-3, Serum paraoxonase/lactonase 3

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  WB analysis using 86577-1-RR (same clone as 86577-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86577-1-RR (Pon3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86577-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02543-1

  Serum of six mice was measured. The PON3 concentration of detected samples was determined to be 19.6 ng/mL with a range of 16.7-21.2 ng/mL.

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  Sandwich ELISA sample test of MP02543-1

  The mean PON3 concentration was determined to be 41.6 ng/mL in RAW 264.7 cell extract based on a 1.3 mg/mL extract load and 1,033.8 ng/mL in mouse liver tissue extract based on a 8.4 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02543-1

  Sandwich ELISA standard curve of MP02543-1, Mouse PON3 Recombinant Matched Antibody Pair - PBS only. 86577-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2743. 86577-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

• 

  Cytometric bead array sample test of MP02543-1

  The mean PON3 concentration was determined to be 986.3 ng/mL in Mouse liver tissue extract based on a 8.4 mg/mL extract load.

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  Cytometric bead array standard curve of MP02543-1

  Cytometric bead array standard curve of MP02543-1, MOUSE PON3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86577-2-PBS. Detection antibody: 86577-1-PBS. Standard: Eg2743. Range: 0.469-60 ng/mL.

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Geprüfte Anwendungen

Produktinformation

86577-1-PBS bindet in WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA Pon3 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Pon3 fusion protein Eg2743
• Vollständiger Name: paraoxonase 3
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: NM_173006.1
• Gene symbol: Pon3
• Gene ID (NCBI): 269823
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PON3 (paraoxonase 3) is a member of the paraoxonase gene family and shares high homology with PON1 and PON2. The PON3 protein is primarily synthesized by the liver and can bind to high-density lipoprotein (HDL), where it exerts antioxidant functions. PON3 can prevent the oxidation of low-density lipoprotein (LDL) and disrupt bacterial quorum-sensing molecules. Additionally, PON3 is expressed in mouse models of Alzheimer's disease (AD), with positive staining for PON3 detected in astrocytes surrounding Aβ plaques. These findings suggest that PON3 plays an important role in antioxidant stress and lipid peroxidation and may have potential protective effects on pathological processes such as neurodegenerative diseases and atherosclerosis.