Prealbumin/transthyretin Monoklonaler Antikörper

Prealbumin/transthyretin Monoklonal Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1H8C4

Kat-Nr. : 66108-1-PBS

Synonyme

Prealbumin, transthyretin, TTR, 1H8C4, ATTR

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  WB analysis of human brain using 66108-1-Ig (same clone as 66108-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66108-1-Ig (same clone as 66108-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66108-1-Ig (same clone as 66108-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66108-1-Ig (same clone as 66108-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66108-1-Ig (Prealbumin/transthyretin antibody at dilution of 1:50. This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-12 using 66108-1-Ig (same clone as 66108-1-PBS)

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Prealbumin/transthyretin (66108-1-Ig, Clone:1H8C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66108-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66108-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA Prealbumin/transthyretin und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Prealbumin/transthyretin fusion protein Ag19232
• Vollständiger Name: transthyretin
• Berechnetes Molekulargewicht: 147 aa, 16 kDa
• Beobachtetes Molekulargewicht: 14-16 kDa, 28-33 kDa
• GenBank-Zugangsnummer: BC020791
• Gene symbol: TTR
• Gene ID (NCBI): 7276
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transthyretin (TTR) is a plasma transport protein for thyroxine and retinol, through the association with retinol-binding protein. It is a homotetrameric protein synthesized mainly in liver, choroid plexus, retinal pigment epithelium, and pancreas.Within the CNS, TTR is the only known protein synthesized solely by the choroid plexus. Mutant and wildtype TTR give rise to various forms of amyloid deposition (amyloidosis). Defects in TTR are the cause of amyloidosis transthyretin-related (AMYL-TTR),hyperthyroxinemia dystransthyretinemic euthyroidal (HTDE) and carpal tunnel syndrome type 1 (CTS1). In addition, positive immunostaining for TTR has been reported as a sensitive diagnostic marker of choroid plexus tumors. TTR can be expressed as monomer (14-16 kDa), dimmer (28-33 kDa), or tetramer around 55-60 kDa. (PMID: 22103483)