Presenilin-1-Specific Polyklonaler Antikörper

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• KD/KO Validated

Presenilin-1-Specific Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16163-1-PBS

Synonyme

PSEN1, AD3, EC:3.4.23.-, Presenilin-1, Presenilin-1 CTF subunit

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  WB analysis using 16163-1-AP (same clone as 16163-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16163-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 16163-1-AP (same clone as 16163-1-PBS)

  IP result of anti-Presenilin-1-Specific (IP:16163-1-AP, 4ug; Detection:16163-1-AP 1:1000) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 16163-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 16163-1-AP (same clone as 16163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16163-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16163-1-AP (same clone as 16163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16163-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 16163-1-AP (same clone as 16163-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16163-1-AP (Presenilin-1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16163-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16163-1-PBS bindet in WB, IHC, IP, Indirect ELISA Presenilin-1-Specific und zeigt Reaktivität mit Affe, human, Maus, Ratten

• Getestete Reaktivität: Affe, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: presenilin 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 21 kDa, 53 kDa
• GenBank-Zugangsnummer: NM_000021
• Gene symbol: PSEN1
• Gene ID (NCBI): 5663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.