Presenilin-1-Specific Rekombinanter Antikörper

Presenilin-1-Specific Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240615B4

Kat-Nr. : 83618-3-RR

Synonyme

PSEN1, Presenilin-1 CTF12, Presenilin-1 CTF subunit, Presenilin-1, EC:3.4.23.-

---

• 

  WB analysis using 83618-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83618-3-RR (PSEN1-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 83618-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 83618-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83618-3-RR (Presenilin-1-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 83618-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of rat brain using 83618-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using Presenilin-1-Specific antibody (83618-3-RR, Clone: 240615B4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Caco-2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83618-3-RR bindet in WB, IHC, IF-P, ELISA Presenilin-1-Specific und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: presenilin 1
• Berechnetes Molekulargewicht: 53 kDa
• Beobachtetes Molekulargewicht: 53 kda
• GenBank-Zugangsnummer: NM_000021
• Gene symbol: PSEN1
• Gene ID (NCBI): 5663
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein.