CALCA/Calcitonin Rekombinanter Antikörper

CALCA/Calcitonin Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240753G2

Kat-Nr. : 83774-1-PBS

Synonyme

Procalcitonin/CALCA, CALCA, Calcitonin, 240753G2, CALC1

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  IF Staining of TT using 83774-1-RR (same clone as 83774-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed TT cells using Procalcitonin/CALCA antibody (83774-1-RR, Clone: 240753G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).(negative control: HeLa cells) This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

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  FC experiment of TT using 83774-1-RR (same clone as 83774-1-PBS)

  1x10^6 TT cells were intracellularly stained with 0.25 ug Procalcitonin/CALCA Recombinant Antibody (83774-1-RR, Clone:240753G2) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83774-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83774-1-PBS

  Biolayer interferometry (BLl) kinetic assay of 83774-1-PBS against Human CALCA/Calcitonin was performed. The affinity constant is 47.7 nM.

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  Affinity and Kinetic Characterization of 83774-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83774-1-RR against Human CALCA/CGRP were performed. The affinity constant is 0.189 nM.

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Geprüfte Anwendungen

Produktinformation

83774-1-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA CALCA/Calcitonin und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CALCA/Calcitonin fusion protein Eg1060
• Vollständiger Name: Calcitonin
• Berechnetes Molekulargewicht: 15kDa
• GenBank-Zugangsnummer: NM_001741.3
• Gene symbol: CALCA
• Gene ID (NCBI): 796
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcitonin is a peptide hormone consisting of 32 amino acids, primarily synthesized by the parafollicular cells (C cells) of the human thyroid gland. It plays a crucial role in regulating calcium levels in the blood by decreasing them. Calcitonin opposes the actions of parathyroid hormone, which increases blood calcium levels. Calcitonin works through a G protein-coupled receptor, known as the calcitonin receptor, which predominantly transmits signals via the cAMP and PLC/IP3 pathways. Its primary physiological effects are on osteoclasts and the tubular epithelium of kidneys.