Prohibitin 2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

Prohibitin 2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1D9C7

Kat-Nr. : 66424-1-PBS

Synonyme

PHB2, 1D9C7, BAP, B-cell receptor-associated protein BAP37, D prohibitin

---

• 

  WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

  WB result of Prohibitin 2 antibody (66424-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66424-1-Ig (same clone as 66424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66424-1-Ig (Prohibitin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66424-1-Ig (Prohibitin 2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). F-actin was labelled in red with Phalloidin. This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 66424-1-Ig (same clone as 66424-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Prohibitin 2 antibody (66424-1-Ig, Clone: 1D9C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66424-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66424-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Prohibitin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Prohibitin 2 fusion protein Ag2977
• Vollständiger Name: prohibitin 2
• Berechnetes Molekulargewicht: 299 aa, 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC014766
• Gene symbol: Prohibitin 2
• Gene ID (NCBI): 11331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)