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  • Featured Product
  • KD/KO Validated

Prohibitin 2 Monoklonaler Antikörper

Prohibitin 2 Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D9C7

Kat-Nr. : CL488-66424

Synonyme

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated Prohibitin 2 Monoclo (CL488-66424)

    IF Staining of HeLa using CL488-66424

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66424 (Prohibitin 2 antibody) at dilution of 1:100.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66424 bindet in IF/ICC Prohibitin 2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Prohibitin 2 fusion protein Ag2977
Vollständiger Name prohibitin 2
Berechnetes Molekulargewicht 299 aa, 33 kDa
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC014766
Gene symbol Prohibitin 2
Gene ID (NCBI) 11331
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL Plus 488 Prohibitin 2 antibody CL488-66424Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Sara (Verified Customer) (10-06-2024)

It works in HEK cells (human), partial colocalisation with mitochondria is seen when using Mitotracker. Very easy staining, as the antibody is already conjugated to Coralite 488. I fixed with 4% PFA, perm and stain overnight.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: HEK
CL Plus 488 Prohibitin 2 Antibody Immunofluorescence validation (1:100 dilution) in HEK (Cat no:CL488-66424)