Prolactin Rekombinanter Antikörper

Prolactin Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241771A5

Kat-Nr. : 85042-1-PBS

Synonyme

241771A5

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  WB analysis using 85042-1-RR (same clone as 85042-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85042-1-RR (Prolactin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide and mouse spleen tissue slide (negative control) using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse pituitary gland using 85042-1-RR (same clone as 85042-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85042-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85042-1-RR against Mouse Prolactin were performed. The affinity constant is 4.50 nM.

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Geprüfte Anwendungen

Produktinformation

85042-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Prolactin und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Prolactin fusion protein Eg0937
• Vollständiger Name: prolactin
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NP_035294.2
• Gene symbol: Prl
• Gene ID (NCBI): 19109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056).