Prolactin Rekombinanter Antikörper

Prolactin Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241771A5

Kat-Nr. : 85042-1-RR

Synonyme

241771A5

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  WB analysis using 85042-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85042-1-RR (Prolactin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pituitary gland using 85042-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pituitary gland using 85042-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse pituitary gland tissue slide and mouse spleen tissue slide (negative control) using 85042-1-RR (Prolactin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pituitary gland using 85042-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse pituitary gland using 85042-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pituitary gland tissue using Prolactin antibody (85042-1-RR, Clone: 241771A5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 85042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85042-1-RR against Mouse Prolactin were performed. The affinity constant is 4.50 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenlungengewebe, Rattenmilzgewebe
• Erfolgreiche Detektion in IHC: mouse pituitary gland tissue, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: mouse pituitary gland tissue

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:2500-1:10000
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85042-1-RR bindet in WB, IHC, IF-P, ELISA Prolactin und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Prolactin fusion protein Eg0937
• Vollständiger Name: prolactin
• Berechnetes Molekulargewicht: 26 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: NP_035294.2
• Gene symbol: Prl
• Gene ID (NCBI): 19109
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056).