Prostein Monoklonaler Antikörper

Prostein Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

5E12E2

Kat-Nr. : 60343-1-PBS

Synonyme

SLC45A3, PRST, Prostate cancer-associated protein 6, PCANAP6, 5E12E2

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  WB analysis using 60343-1-Ig (same clone as 60343-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 60343-1-Ig (same clone as 60343-1-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 60343-1-Ig (same clone as 60343-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 60343-1-Ig (Prostein antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 60343-1-Ig (Prostein antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60343-1-Ig (Prostein antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate cancer using 60343-1-Ig (same clone as 60343-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate cancer tissue using Prostein antibody (60343-1-Ig, Clone: 5E12E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60343-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

60343-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Prostein und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Prostein fusion protein Ag5463
• Vollständiger Name: solute carrier family 45, member 3
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 55-59 kDa
• GenBank-Zugangsnummer: BC050416
• Gene symbol: Prostein
• Gene ID (NCBI): 85414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Prostein is a prostate tissue-specific protein whose expression is highly restricted to normal and cancerous prostate tissues. Anti-prostein is useful for the identification of prostate adenocarcinomas and is a good marker to identify prostatic origin in metastatic cancer.