Protein A Monoklonaler Antikörper

Protein A Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Staphylococcus aureus und mehr (1)

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1G6A6

Kat-Nr. : 66945-1-PBS

Synonyme

---

• 

  WB analysis of Recombinant protein using 66945-1-Ig (same clone as 66945-1-PBS)

  Commercial Protein A was deactivated and lysised followed by SDS-PAGE and then blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 and isotype control antibody 66360-1-Ig at 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66945-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ProteinA using 66945-1-Ig (same clone as 66945-1-PBS)

  Commercial Protein A and negative samples (human plasma, HeLa cells, rat liver, mouse brain) were subjected to SDS-PAGE followed by western blot with 66945-1-Ig (Protein A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66945-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66945-1-PBS bindet in WB, Indirect ELISA Protein A und zeigt Reaktivität mit Staphylococcus aureus

• Getestete Reaktivität: Staphylococcus aureus
• In Publikationen genannte Reaktivität: Hefe
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: Protein A
• GenBank-Zugangsnummer: EU695225
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein A is a surface protein of S.aureus which binds IgG molecules by their Fc region (PMID: 4163007). Engineered Protein A has been widely used in antibody purification due to its Fc binding property. However, trace of protein A might be leaked from purification resin thus causes contamination of final products. Antibody against Protein A or ELISA kits for Protein A could be used to evaluate leaking level of Protein A from resin. Protein A should be deactivated (e.g. boiling in 5-10% tween-20) before testing.

Publikationen

Species	Application	Title
yeast	WB	Proc Natl Acad Sci U S A; Mec1-mediated Atg9 phosphorylation regulates the PAS recruitment of Atg9 vesicles upon energy stress; Authors - Siyu Fan