Coagulation factor II Rekombinanter Antikörper

Coagulation factor II Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243089F11

Kat-Nr. : 86124-1-PBS

Synonyme

F2, Prothrombin, Activation peptide fragment 1, Activation peptide fragment 2, EC:3.4.21.5

---

• 

  WB analysis of human plasma using 86124-1-RR (same clone as 86124-1-PBS)

  human plasma were subjected to SDS PAGE followed by western blot with 86124-1-RR (Prothrombin/F2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86124-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array sample test of MP02277-1

  The mean Coagulation Factor II/F2 concentration was determined to be 986.4 ug/mL in human serum.

• 

  Cytometric bead array standard curve of MP02277-1

  Cytometric bead array standard curve of MP02277-1, Coagulation Factor II/F2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86124-2-PBS. Detection antibody: 86124-1-PBS. Standard: Eg1337. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 86124-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86124-1-RR against Human Coagulation factor II were performed. The affinity constant is 54.6 nM.

---

Geprüfte Anwendungen

Produktinformation

86124-1-PBS bindet in WB, Cytometric bead array, Indirect ELISA Coagulation factor II und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Coagulation factor II fusion protein Eg1337
• Vollständiger Name: coagulation factor II (prothrombin)
• Berechnetes Molekulargewicht: 70kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: NM_000506.5
• Gene symbol: F2
• Gene ID (NCBI): 2147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II (F2), also known as prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.