Pseudouridine Monoklonaler Antikörper

Pseudouridine Monoklonal Antikörper für Dot Blot, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

chemical compound und mehr (1)

Anwendung

Dot Blot, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

1G7C7

Kat-Nr. : 68578-1-Ig

Synonyme

Ψ, 5-ribosyluracil

---

• 

  ELISA experiment of Pseudouridine using 68578-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to Pseudouridine. Indirect ELISA was performed by coating BSA conjugated Pseudouridine at 10ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection. Competitive ELISA was performed similarly except that different concentration of Pseudouridine or its structure analogue compounds are mixed in primary antibody (fixed at 10 ng/mL).

• 

  Dot Blot experiment of RNA using 68578-1-Ig

  Total RNA was isolated from HeLa cell line and was dotted to NC membrane at different amount as indicated above the dots. The membrane was blocked with BSA and blotted with Pseudouridine antibody 68578-1-Ig at 1:2000 followed by incubation of HRP-goat anti-mouse secondary antibody. Signal was developed by ECL substrate. A parallel dot blot was performed using mouse IgG1 isotype control antibody 66360-1-Ig at the same dose.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in ELISA: Pseudouridine
• Erfolgreiche Detektion in Dot Blot: RNA

Empfohlene Verdünnung

• Enzyme-linked Immunosorbent Assay (ELISA): ELISA : 1:2000-1:20000
• DOT BLOT: DOT BLOT : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68578-1-Ig bindet in Dot Blot, ELISA Pseudouridine und zeigt Reaktivität mit chemical compound

• Getestete Reaktivität: chemical compound
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PTG
• Vollständiger Name: Pseudouridine
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pseudouridine is an isomer of the nucleoside uridine in which the uracil is attached via a carbon-carbon instead of a nitrogen-carbon glycosidic bond. Pseudouridine is the most abundant RNA modification in cellular RNA (PMID: 29104216). After transcription and following synthesis, RNA can be modified with over 100 chemically distinct modifications. These can potentially regulate RNA expression post-transcriptionally, in addition to the four standard nucleotides and play a variety of roles in the cell including translation, localization and stabilization of RNA. Pseudouridine in rRNA and tRNA has been shown to fine-tune and stabilize the regional structure and help maintain their functions in mRNA decoding, ribosome assembly, processing and translation (PMID: 10902565, PMID: 23391857, PMID: 28045575). Pseudouridine in snRNA has been shown to enhance spliceosomal RNA-pre-mRNA interaction to facilitate splicing regulation (PMID 27268497).

Protocol for Dot Blot:
https://www.ptglab.com/protocol/68578-1-IgDotBlot.pdf

Publikationen

Species	Application	Title
human		Cell Signal; PUS1 facilitates cell migration in clear cell renal cell carcinoma through the promotion of mRNA pseudouridylation and the stabilization of the SMOX gene; Authors - Yao Chen
		Gastroenterol Rep (Oxf); TRUB1 is a novel biomarker for promoting malignancy in colorectal cancer via NFκB signaling; Authors - Yingzhao Wang