GlnRS Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

GlnRS Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, RIP, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12645-1-AP

Synonyme

GlnRS, Glutamine tRNA ligase, glutaminyl tRNA synthetase, PRO2195, QARS

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Galerie der Validierungsdaten

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  WB analysis using 12645-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12645-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12645-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12645-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12645-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12645-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12645-1-AP (GlnRS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 12645-1-AP

  IP Result of anti-GlnRS (IP:12645-1-AP, 4ug; Detection:12645-1-AP 1:500) with PC-3 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 12645-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12645-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12645-1-AP (GlnRS antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 12645-1-AP

  Immunofluorescent analysis of PC-3 cells using 12645-1-AP (GlnRS antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, PC-3-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:1500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• RIP: See 1 publications below

Produktinformation

12645-1-AP bindet in WB, RIP, IP, IHC, IF, ELISA GlnRS und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: GlnRS fusion protein Ag3375
• Vollständiger Name: glutaminyl-tRNA synthetase
• Berechnetes Molekulargewicht: 775 aa, 88 kDa
• Beobachtetes Molekulargewicht: 88 kDa
• GenBank-Zugangsnummer: BC016634
• Gene symbol: QARS
• Gene ID (NCBI): 5859
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLnRS(glutaminyl-tRNA synthetase) is a class I aminoacyl-tRNA synthetase. Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. The specificity of this reaction determines the fidelity of mRNA translation. At least 1 synthetase exists in the cytoplasm for each amino acid.GlnRS is present in eukaryotes but is absent from many prokaryotes, mitochondria, and chloroplasts, in which Gln-tRNA(Gln) is formed by transamidation of the misacylated Glu-tRNA(Gln)(PMID:8078941).

Publikationen

Species	Application	Title
human	WB	Nat Cell Biol; Leucyl-tRNA synthetase is a tumour suppressor in breast cancer and regulates codon-dependent translation dynamics.; Authors - Maria C Passarelli
human	WB	Nucleic Acids Res; Regulation of ex-translational activities is the primary function of the multi-tRNA synthetase complex.; Authors - Haissi Cui
human	WB,RIP	EMBO Rep; The tRNA-like small noncoding RNA mascRNA promotes global protein translation.; Authors - Xinping Lu ; KD Validated