QPCT Polyklonaler Antikörper

QPCT Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18571-1-AP

Synonyme

EC, EC:2.3.2.5, Glutaminyl cyclase, glutaminyl peptide cyclotransferase, Glutaminyl-peptide cyclotransferase

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  WB analysis using 18571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18571-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18571-1-AP (QPCT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 18571-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using QPCT antibody (18571-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Caki-2 cells, humane Urinprobe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18571-1-AP bindet in WB, IF/ICC, ELISA QPCT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: QPCT fusion protein Ag13416
• Vollständiger Name: glutaminyl-peptide cyclotransferase
• Berechnetes Molekulargewicht: 361 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa, 35 kDa
• GenBank-Zugangsnummer: BC047756
• Gene symbol: QPCT
• Gene ID (NCBI): 25797
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutaminyl-peptide cyclotransferase (QPCT) gene encodes glutaminyl cyclase (QC), an enzyme that plays a role in post-translational modification of proteins by converting N-terminal glutamate to pyroglutamine. QPCT plays a specific role in promoting tumor cell proliferation, migration and angiogenesis as well as in inhibiting tumor cell apoptosis in cancers such as thyroid cancer and renal cell carcinoma (PMID: 38417050). Human QPCT has two isoforms produced by alternative splicing, 41 kDa and 35 kDa, respectively.