QRICH1 Polyklonaler Antikörper

QRICH1 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30236-1-AP

Synonyme

FLJ20259, glutamine rich 1, Glutamine rich protein 1, QRICH1

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Galerie der Validierungsdaten

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  WB analysis using 30236-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30236-1-AP (QRICH1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 30236-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30236-1-AP (QRICH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 30236-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 30236-1-AP (QRICH1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 30236-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using QRICH1 antibody (30236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Jurkat-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, Maushirngewebe, Maus-Magengewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30236-1-AP bindet in WB, IHC, IF, ELISA QRICH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: QRICH1 fusion protein Ag31997
• Vollständiger Name: glutamine-rich 1
• Beobachtetes Molekulargewicht: 85-87 kDa
• GenBank-Zugangsnummer: BC110855
• Gene symbol: QRICH1
• Gene ID (NCBI): 54870
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Glutamine-rich protein 1 (QRICH1) is a transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PMID:33384352). QRICH1 is a member of the caspase recruitment domain (CARD)-containing gene family, which encodes for proteins implicated in regulation of caspase activation in the context of apoptosis and inflammation, and in regulation of NF-κB signaling(PMID:33384352). QRICH1 can be modified by phosphorylation.