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RAB10 Rekombinanter Antikörper

RAB10 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251744G12

Kat-Nr. : 86787-1-PBS

Synonyme

EC:3.6.5.2, Ras related protein Rab 10, Ras-related protein Rab-10

Filter:
  • Western Blot (WB) analysis of various lysates using RAB10 Recombinant monoclonal antibody (86787-1-RR)

    WB analysis using 86787-1-RR (same clone as 86787-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86787-1-RR (RAB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86787-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RAB10 Recombinant monoclonal antibody (86787-1-RR)

    IF Staining of MCF-7 using 86787-1-RR (same clone as 86787-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using RAB10 antibody (86787-1-RR, Clone: 251744G12 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86787-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86787-1-RR

    Affinity and Kinetic Characterization of 86787-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86787-1-RR against Human RAB10 were performed. The affinity constant is 3.91 nM.

Geprüfte Anwendungen

Produktinformation

86787-1-PBS bindet in WB, IF/ICC, Indirect ELISA RAB10 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RAB10 fusion protein Ag25830
Vollständiger Name RAB10, member RAS oncogene family
Berechnetes Molekulargewicht 200 aa, 23 kDa
Beobachtetes Molekulargewicht20-23 kDa
GenBank-ZugangsnummerBC000896
Gene symbol RAB10
Gene ID (NCBI) 10890
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB10 is a member of the RAB family of small GTPases, which are key regulators of vesicular trafficking. RAB proteins are cyclically controlled, requiring a GDP-GTP exchange that is facilitated by RAB guanine nucleotide exchange factors. Rab10 plays essential functional roles in development: mice are embryonic lethal when both alleles are deleted. In addition, RAB10 plays a role in maintenance and regulation of endoplasmic reticulum (ER) morphology. RAB10 function has also been linked to neuronal morphology and polarization, playing a critical role in axonal development and dendrite arborization.