RAB11FIP2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB11FIP2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 18136-1-AP

Synonyme

NRip11, Rab11 family-interacting protein 2, Rab11 FIP2, Rab11-FIP2

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  WB analysis using 18136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18136-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18136-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18136-1-AP (RAB11FIP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 18136-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB11FIP2 antibody (18136-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 18136-1-AP

  Immunofluorescent analysis of HepG2 cells, using RAB11FIP2 antibody 18136-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, Mauslebergewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 2 publications below

Produktinformation

18136-1-AP bindet in WB, IF/ICC, ELISA RAB11FIP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB11FIP2 fusion protein Ag12736
• Vollständiger Name: RAB11 family interacting protein 2 (class I)
• Berechnetes Molekulargewicht: 512 aa, 58 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC075073
• Gene symbol: RAB11FIP2
• Gene ID (NCBI): 22841
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB11FIP2, or RAB11 family interacting protein 2, is a protein that plays a crucial role in intracellular trafficking, particularly in the recycling of endocytic vesicles. It is involved in several cellular processes, including TRAM-dependent toll-like receptor 4 signaling pathway, phagocytosis, and positive regulation of GTPase activity. RAB11FIP2 is localized in endosomes, nucleoplasm, and phagocytic cups, and it is expressed ubiquitously in various tissues, with higher expression in endometrium and brain. RAB11FIP2 interacts with the actin-based motor myo5b and the small GTPase Rab11, which are key components in the regulation of vesicle tethering and transport along actin filaments. This interaction is particularly important for processes such as cell polarization, motility, and lumen formation in multicellular structures. .

Publikationen

Species	Application	Title
human	WB,IF	PLoS Pathog; Acquisition of Rab11 and Rab11-Fip2-A novel strategy for Chlamydia pneumoniae early survival.; Authors - Katja Mölleken ; KD Validated
human	WB	Traffic; Rab11-FIP2 Interaction with MYO5B Regulates Movement of Rab11a-Containing Recycling Vesicles.; Authors - Jenny C Schafer
human	IF	Exp Cell Res; Rab11-FIP1A regulates early trafficking into the recycling endosomes.; Authors - Jenny C Schafer
human	WB	Cell Biosci; Peptides targeting RAB11A-FIP2 complex inhibit HPIV3, RSV, and IAV replication as broad-spectrum antivirals; Authors - Yanliang Jiang