RAB11FIP5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RAB11FIP5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 14594-1-AP

Synonyme

GAF1, Gaf-1, Gamma-SNAP-associated factor 1, Rab11 family-interacting protein 5, Rab11-interacting protein Rip11

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 14594-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, HeLa-Zellen, Jurkat-Zellen, Mausherzgewebe, Mausnierengewebe, Mauslungengewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, Maus-Kolongewebe, Mausnierengewebe, Ratten-Kolongewebe, Rattennierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 3 publications below

Produktinformation

14594-1-AP bindet in WB, IHC, IF, ELISA RAB11FIP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB11FIP5 fusion protein Ag6128
• Vollständiger Name: RAB11 family interacting protein 5 (class I)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC035013
• Gene symbol: RAB11FIP5
• Gene ID (NCBI): 26056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.; Authors - Kai He ; KD Validated
mouse,human	WB,IF	J Extracell Vesicles; Breast Cancer-Derived Extracellular Vesicles Modulate the Cytoplasmic and Cytoskeletal Dynamics of Blood-Brain Barrier Endothelial Cells; Authors - Sara Busatto
	IF	J Cell Sci; Proximity labelling identifies pro-migratory endocytic recycling cargo and machinery of the Rab4 and Rab11 families; Authors - Beverley Wilson