RAB11FIP5 Polyklonaler Antikörper

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• KD/KO Validated

RAB11FIP5 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 14594-1-AP

Synonyme

Gaf 1, GAF1, Gamma SNAP associated factor 1, KIAA0857, Phosphoprotein pp75, pp75, Rab11 FIP5, RAB11FIP5, RIP11

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Galerie der Validierungsdaten

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  WB analysis of mouse kidney using 14594-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14594-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14594-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 14594-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14594-1-AP (RAB11FIP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293T-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Herzgewebe, Mausherzgewebe, Mauslungengewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

14594-1-AP bindet in WB, IF, ELISA RAB11FIP5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB11FIP5 fusion protein Ag6128
• Vollständiger Name: RAB11 family interacting protein 5 (class I)
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC035013
• Gene symbol: RAB11FIP5
• Gene ID (NCBI): 26056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rab11 family-interacting protein 5 (Rab11fip5) is an adaptor protein that binds to the small GTPase Rab11, which has an important function in endosome recycling and trafficking of cellular proteins to the plasma membrane. Rab11fip5 is involved in many cellular processes, such as cytoskeleton rearrangement, iron uptake and exocytosis in neuroendocrine cells, and is also known as a candidate gene for autism-spectrum disorder.

Publikationen

Species	Application	Title
human	WB,IF	Nat Commun; Axoneme polyglutamylation regulated by Joubert syndrome protein ARL13B controls ciliary targeting of signaling molecules.; Authors - Kai He ; KD Validated