RAB12 Polyklonaler Antikörper

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• KD/KO Validated

RAB12 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 18843-1-AP

Synonyme

RAB12, Ras related protein Rab 12

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Galerie der Validierungsdaten

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  WB analysis using 18843-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18843-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18843-1-AP (RAB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18843-1-AP

  IP Result of anti-RAB12 (IP:18843-1-AP, 4ug; Detection:18843-1-AP 1:500) with HeLa cells lysate 1200ug.

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  IHC staining of human kidney using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18843-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18843-1-AP (RAB12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18843-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB12 antibody (18843-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 12 publications below
• IP: See 1 publications below

Produktinformation

18843-1-AP bindet in WB, IP, IHC, IF, ELISA RAB12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: RAB12, member RAS oncogene family
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: NM_001025300
• Gene symbol: RAB12
• Gene ID (NCBI): 201475
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB12 belongs to the small GTPase superfamily and Rab family. RAB12 locates in Golgi apparatus membrane.

Publikationen

Species	Application	Title
human	WB	Curr Biol; Increased LRRK2 kinase activity alters neuronal autophagy by disrupting the axonal transport of autophagosomes.; Authors - C Alexander Boecker
human	WB	EMBO Rep; Phosphorylation of the exchange factor DENND3 by ULK in response to starvation activates Rab12 and induces autophagy.; Authors - Jie Xu
human	WB	Neurobiol Dis; Directing LRRK2 to membranes of the endolysosomal pathway triggers RAB phosphorylation and JIP4 recruitment.; Authors - Jillian H Kluss
mouse	WB	J Immunol; Rab12 Regulates Retrograde Transport of Mast Cell Secretory Granules by Interacting with the RILP-Dynein Complex.; Authors - Adi Efergan ; KD Validated
human	WB	Front Oncol; Rab12 Promotes Radioresistance of HPV-Positive Cervical Cancer Cells by Increasing G2/M Arrest.; Authors - Yujie Huang ; KD Validated
human	WB	Traffic; Rab12 localizes to Shiga toxin-induced plasma membrane invaginations and controls toxin transport.; Authors - Gustaf E Rydell ; KD Validated