RAB13 Rekombinanter Antikörper

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• KD/KO Validated

RAB13 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240216D10

Kat-Nr. : 83336-2-PBS

Synonyme

GIG4, 240216D10, Cell growth inhibiting gene 4 protein, Cell growth-inhibiting gene 4 protein, Ras related protein Rab 13

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  WB analysis using 83336-2-RR (same clone as 83336-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83336-2-RR (RAB13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 83336-2-RR (same clone as 83336-2-PBS)

  WB result of RAB13 antibody (83336-2-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RAB13 transfected HEK-293 cells. This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83336-2-RR (same clone as 83336-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RAB13 antibody (83336-2-RR, Clone: 240216D10 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 83336-2-RR (same clone as 83336-2-PBS)

  1x10^6 K-562 cells were intracellularly stained with 0.25 ug RAB13 Recombinant antibody (83336-2-RR, Clone:240216D10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83336-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00354-1

  Cytometric bead array standard curve of MP00354-1, RAB13 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83336-2-PBS. Detection antibody: 83336-1-PBS. Standard: Ag34898. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 83336-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83336-2-RR against Human RAB13 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83336-2-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA RAB13 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RAB13 fusion protein Ag34898
• Vollständiger Name: RAB13, member RAS oncogene family
• Berechnetes Molekulargewicht: 203 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC000799
• Gene symbol: RAB13
• Gene ID (NCBI): 5872
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB13 (Ras-related protein Rab-13) is also named as GIG4. Rab13 is a member of the Rab GTPase family. RAB13 is highly expressed in malignant cells. Rab13 was highly expressed in BCSC of triple-negative breast cancer (TNBC) (PMID: 35395074). RAB13 is involved in a number of processes in LIHC, including MTORC1 signaling, MYC targets v1, G2M checkpoint, MITOTIC spindle, DNA repair, P53 pathway, glycolysis, PI3K-AKT-MTOR signaling, etc (PMID: 37803063). RAB13 enhances its circulation to the plasma membrane to promote breast cancer invasion. By influencing the tumor microenvironment, RAB13 maintains breast cancer stem cells (PMID: 37803063).