RAB14 Polyklonaler Antikörper

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• KD/KO Validated

RAB14 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 15662-1-AP

Synonyme

EC:3.6.5.2, FBP, RAB 14, Ras related protein Rab 14, Ras-related protein Rab-14

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  WB analysis of mouse brain using 15662-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15662-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 15662-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 15662-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15662-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 15662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 15662-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen, humanes Hirngewebe, Mausnierengewebe, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 11 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Produktinformation

15662-1-AP bindet in WB, IHC, IF, ELISA RAB14 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB14 fusion protein Ag8092
• Vollständiger Name: RAB14, member RAS oncogene family
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC006081
• Gene symbol: RAB14
• Gene ID (NCBI): 51552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB14, the final member of the Rab11 subfamily, participates in the regulation of cell secretion, ensuring the timely release of necessary substances outside the cell. RAB14 is ubiquitously expressed and localizes to the Golgi/TGN and at early endosomes (PMID: 16962593, 22595670). RAB14 contains 215 amino acids, with a molecular weight of approximately 24.6 kDa

Publikationen

Species	Application	Title
human	WB,IHC	Cell Physiol Biochem; Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer.; Authors - Bo Guo
human	WB,IHC	Onco Targets Ther; Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers.; Authors - Jun Zhang
human	WB	PLoS One; Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.; Authors - Bo Guo
human	WB,IHC	Virchows Arch; PMID: 30267303; Authors - Jinnian Ge ; KD Validated
human	WB,IHC	Tumour Biol; Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway.; Authors - Rui Hou ; KD Validated
human	WB	Eur Rev Med Pharmacol Sci; STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14.; Authors - C Du