RAB14 Monoklonaler Antikörper

RAB14 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G7B3

Kat-Nr. : 67953-1-Ig

Synonyme

3G7B3, EC:3.6.5.2, FBP, RAB 14, Ras related protein Rab 14

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  WB analysis using 67953-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

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  WB analysis of pig brain using 67953-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67953-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HCT 116 using 67953-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 67953-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67953-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 67953-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67953-1-Ig (RAB14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse ovary using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 67953-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 67953-1-Ig (RAB14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67953-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RAB14 antibody (67953-1-Ig, Clone: 3G7B3 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: LNCaP-Zellen, HCT 116-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maus-Eierstockgewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67953-1-Ig bindet in WB, IHC, IF/ICC, ELISA RAB14 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RAB14 fusion protein Ag8167
• Vollständiger Name: RAB14, member RAS oncogene family
• Berechnetes Molekulargewicht: 215 aa, 24 kDa
• Beobachtetes Molekulargewicht: 24 kDa
• GenBank-Zugangsnummer: BC006081
• Gene symbol: RAB14
• Gene ID (NCBI): 51552
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB14, the final member of the Rab11 subfamily, participates in the regulation of cell secretion, ensuring the timely release of necessary substances outside the cell. RAB14 is ubiquitously expressed and localizes to the Golgi/TGN and at early endosomes (PMID: 16962593, 22595670). RAB14 contains 215 amino acids, with a molecular weight of approximately 24.6 kDa