RAB17 Polyklonaler Antikörper

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• KD/KO Validated

RAB17 Polyklonal Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 17501-1-AP

Synonyme

Ras-related protein Rab-17, Ras related protein Rab 17

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  IHC staining of human pancreas using 17501-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17501-1-AP (RAB17 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human pancreas using 17501-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17501-1-AP (RAB17 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of WIF-B cells using 17501-1-AP

  IF result of RAB17 antibody (17501-1-AP) with WIF-B cells overexpressed myc-tagged RAB17 by A.C. Striz and Dr. P.L. Tuma.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: WIF-B-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:25-1:50

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

17501-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA RAB17 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RAB17 fusion protein Ag11688
• Vollständiger Name: RAB17, member RAS oncogene family
• Berechnetes Molekulargewicht: 212 aa, 23 kDa
• GenBank-Zugangsnummer: BC050426
• Gene symbol: RAB17
• Gene ID (NCBI): 64284
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RAB17 is a member of the RAS oncogene family and is classified as a small GTPase that plays a crucial role in intracellular membrane trafficking It is specifically expressed in epithelial cells and is involved in various cellular functions such as transcytosis, the directed movement of endocytosed material through the cell and its exocytosis from the plasma membrane at the opposite side. This process is mainly observed in epithelial cells and is important for the transcellular transport of substances like immunoglobulins from the basolateral surface to the apical surface. RAB17 is also implicated in the regulation of melanosome transport and release from melanocytes, which is critical for pigmentation in skin cells . Additionally, it has been suggested to regulate dendrite and dendritic spine development, which are important for neuronal function. The protein is involved in membrane trafficking through apical recycling endosomes in a post-endocytic step of transcytosis.

Publikationen

Species	Application	Title
human	WB,IF	Mol Cancer; Intercellular transfer of exosomal wild type EGFR triggers osimertinib resistance in non-small cell lung cancer.; Authors - Shaocong Wu ; KD Validated
human	WB,IHC,IF,CoIP	Cell Death Dis; RAB17 promotes endometrial cancer progression by inhibiting TFRC-dependent ferroptosis; Authors - Xing Zhou ; KD Validated
human	IHC	Int J Mol Sci; The Tolerogenic Influence of Dexamethasone on Dendritic Cells Is Accompanied by the Induction of Efferocytosis, Promoted by MERTK; Authors - Vivien Li
human	IF	Cell Death Dis; Rab17 mediates differential antigen sorting following efferocytosis and phagocytosis.; Authors - Charles Yin
	WB	J Biol Chem; The GTP-bound and Sumoylated Form of the rab17 Small Molecular Weight GTPase Selectively Binds Syntaxin 2 in Polarized Hepatic WIF-B Cells.; Authors - Anneliese C Striz
	WB	Mol Biol Cell; Rab17 Regulates apical delivery of hepatic transcytotic vesicles.; Authors - Anneliese C Striz